Sum Frequency Generation Confocal Microscopy Observation of a Fish Scale
نویسندگان
چکیده
منابع مشابه
Polarization-sensitive sum-frequency generation microscopy of collagen fibers.
Point-scanning sum-frequency generation (SFG) microscopy enables the generation of images of collagen I fibers in tissues by tuning into specific vibrational resonances of the polypeptide. It is shown that when collagen-rich tissues are visualized near the 2954 cm(-1) stretching vibration of methylene groups, the SFG image contrast is higher compared to the contrast seen in nonresonant second-h...
متن کاملRapid vibrational imaging with sum frequency generation microscopy.
We demonstrate rapid vibrational imaging based on sum frequency generation (SFG) microscopy with a collinear excitation geometry. Using the tunable picosecond pulses from a high-repetition-rate optical parametric oscillator, vibrationally selective imaging of collagen fibers is achieved with submicrometer lateral resolution. We furthermore show simultaneous SFG and second harmonic generation im...
متن کاملAdvances in Vibrationally Resonant Sum-Frequency Generation Microscopy
Vibrationally resonant sum-frequency generation microscopy is a member of the family of nonlinear optical microscopy techniques. It enables chemically selective imaging of non-centrosymmetric structures. We present the latest advances in this field. OCIS codes: 180.4315, 190.4223 1. The emerging field of sum-frequency generation microscopy Second-harmonic generation (SHG) is a second-order opti...
متن کاملOptically Active Sum Frequency Generation Microscopy for Cellular Imaging
Optically active sum-frequency generation microscopy was applied to imaging cells, utilizing the intrinsic chirality of biological macromolecules as the contrast mechanism.
متن کاملHyperspectral imaging with laser-scanning sum-frequency generation microscopy.
Vibrationally sensitive sum-frequency generation (SFG) microscopy is a chemically selective imaging technique sensitive to non-centrosymmetric molecular arrangements in biological samples. The routine use of SFG microscopy has been hampered by the difficulty of integrating the required mid-infrared excitation light into a conventional, laser-scanning nonlinear optical (NLO) microscope. In this ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: e-Journal of Surface Science and Nanotechnology
سال: 2014
ISSN: 1348-0391
DOI: 10.1380/ejssnt.2014.259